Review



e9h80  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc e9h80
    E9h80, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+v5+tag/V5-Tag+Mouse+mAb/pm42805951-315-26-27
    Average 95 stars, based on 72 article reviews
    e9h80 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    other:

    Article Title: EGFR inhibitor-resistant lung cancers exhibit collateral sensitivity to a covalent, cysteine-independent KEAP1 oligomerizing molecular bridge.
    Article Snippet: Samples were diluted to equal concentrations using lysis buffer and prepared for electrophoresis with NuPAGE LDS Sample Buffer (4X) before boiling at either 70 °C for 10 minutes or 95 °C for 15 minutes.

    SDS Page:

    Article Title: Restoration of RBM22 overcomes the transcriptional and epigenetic barriers of cardiomyocyte proliferation for heart regeneration.
    Article Snippet: Protein concentrations were AR TI CL E IN P RE SS determined using the BCA protein assay kit (Thermo Fisher Scientific, 23227). .. Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Cytiva, 10600002), which were blocked with 5% non-fat dry milk for 1 h. Membranes were incubated overnight at 4°C with primary antibodies: anti-RBM22 (Proteintech, 22103-1-AP, 1: 1000), anti-CDK4 (Proteintech,11026-1-AP, 1: 1000), anti-CCNA2 (Proteintech, 18202-1-AP, 1: 1000), antiCCNE1 (Proteintech, 11554-1-AP, 1: 1000), anti-PCNA (Proteintech, 10205-2-AP, 1: 1000), anti-SMARCA4 (Abcam, ab110641, 1: 1000), anti-SMARCC1 (Abcam, ab305037, 1: 1000), anti-SMARCC2 (Abways, AY4215, 1: 1000), anti-Lamin A/C (Thermo Fisher Scientific, PA517113, 1: 1000), anti-Histone H3 (Cell Signaling Technology, #9715S, 1: 1000), anti-β-Actin (Cell Signaling Technology, #3700, 1: 2000), anti-GAPDH (Cell Signaling Technology, #5174, 1: 2000), anti-Flag-tag (Cell Signaling Technology, #2044, 1: 1000), and anti-V5-tag (Cell Signaling Technology, #13202, 1: 1000). ..

    Incubation:

    Article Title: Restoration of RBM22 overcomes the transcriptional and epigenetic barriers of cardiomyocyte proliferation for heart regeneration.
    Article Snippet: Protein concentrations were AR TI CL E IN P RE SS determined using the BCA protein assay kit (Thermo Fisher Scientific, 23227). .. Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Cytiva, 10600002), which were blocked with 5% non-fat dry milk for 1 h. Membranes were incubated overnight at 4°C with primary antibodies: anti-RBM22 (Proteintech, 22103-1-AP, 1: 1000), anti-CDK4 (Proteintech,11026-1-AP, 1: 1000), anti-CCNA2 (Proteintech, 18202-1-AP, 1: 1000), antiCCNE1 (Proteintech, 11554-1-AP, 1: 1000), anti-PCNA (Proteintech, 10205-2-AP, 1: 1000), anti-SMARCA4 (Abcam, ab110641, 1: 1000), anti-SMARCC1 (Abcam, ab305037, 1: 1000), anti-SMARCC2 (Abways, AY4215, 1: 1000), anti-Lamin A/C (Thermo Fisher Scientific, PA517113, 1: 1000), anti-Histone H3 (Cell Signaling Technology, #9715S, 1: 1000), anti-β-Actin (Cell Signaling Technology, #3700, 1: 2000), anti-GAPDH (Cell Signaling Technology, #5174, 1: 2000), anti-Flag-tag (Cell Signaling Technology, #2044, 1: 1000), and anti-V5-tag (Cell Signaling Technology, #13202, 1: 1000). ..

    Article Title: Aerosolized adenoviral IL4/IL10 delivery alleviates LPS-induced acute lung injury.
    Article Snippet: .. After blocking with 5 % BSA in TBST, the membranes were incubated overnight at 4 ◦C with the following primary antibodies: anti-Myc tag (1:1000, #2272, Cell Signaling Technology), anti-V5 tag (1:1000, #13,202, Cell Signaling Technology), and anti-β-actin (1:1000, AF0003, Beyotime). .. Following three washes with TBST, the membranes were incubated at room temperature for 1 h with the corresponding HRP-conjugated secondary antibodies: anti-mouse (1:2000, A0216, Beyotime) or anti-rabbit (1:2000, A0208, Beyotime) on a shaking platform.

    Blocking Assay:

    Article Title: Aerosolized adenoviral IL4/IL10 delivery alleviates LPS-induced acute lung injury.
    Article Snippet: .. After blocking with 5 % BSA in TBST, the membranes were incubated overnight at 4 ◦C with the following primary antibodies: anti-Myc tag (1:1000, #2272, Cell Signaling Technology), anti-V5 tag (1:1000, #13,202, Cell Signaling Technology), and anti-β-actin (1:1000, AF0003, Beyotime). .. Following three washes with TBST, the membranes were incubated at room temperature for 1 h with the corresponding HRP-conjugated secondary antibodies: anti-mouse (1:2000, A0216, Beyotime) or anti-rabbit (1:2000, A0208, Beyotime) on a shaking platform.

    Chromatin Immunoprecipitation:

    Article Title: And-1 coordinates with polymerase δ to regulate nucleotide excision repair and UVB-induced skin tumorigenesis
    Article Snippet: .. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-β-Actin (CST #3700; 1:5000); Anti-GAPDH (CST #2118; 1:5000); Anti-FLAG-tag (CST #14793; 1:1000); Anti-V5-tag (CST #13202; 1:1000); Anti-Histone H3 (CST #9715; 1:1000); Anti-p125 (sc-17776; 1:1000); Anti-PCNA (sc-56; 1:1000); Anti-p66 (#21935-1-AP; 1:1000); Anti-DDB1 (A14242; 1:1000); Anti-CPDs (CAC-NM-DND-001; 1:1000); Anti-6-4PPs (CAC-NM-DND-002; 1:1000); Validation All the antibodies listed above were validated in western blotting. ..

    Flow Cytometry:

    Article Title: And-1 coordinates with polymerase δ to regulate nucleotide excision repair and UVB-induced skin tumorigenesis
    Article Snippet: .. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-β-Actin (CST #3700; 1:5000); Anti-GAPDH (CST #2118; 1:5000); Anti-FLAG-tag (CST #14793; 1:1000); Anti-V5-tag (CST #13202; 1:1000); Anti-Histone H3 (CST #9715; 1:1000); Anti-p125 (sc-17776; 1:1000); Anti-PCNA (sc-56; 1:1000); Anti-p66 (#21935-1-AP; 1:1000); Anti-DDB1 (A14242; 1:1000); Anti-CPDs (CAC-NM-DND-001; 1:1000); Anti-6-4PPs (CAC-NM-DND-002; 1:1000); Validation All the antibodies listed above were validated in western blotting. ..

    Magnetic Resonance Imaging:

    Article Title: And-1 coordinates with polymerase δ to regulate nucleotide excision repair and UVB-induced skin tumorigenesis
    Article Snippet: .. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-β-Actin (CST #3700; 1:5000); Anti-GAPDH (CST #2118; 1:5000); Anti-FLAG-tag (CST #14793; 1:1000); Anti-V5-tag (CST #13202; 1:1000); Anti-Histone H3 (CST #9715; 1:1000); Anti-p125 (sc-17776; 1:1000); Anti-PCNA (sc-56; 1:1000); Anti-p66 (#21935-1-AP; 1:1000); Anti-DDB1 (A14242; 1:1000); Anti-CPDs (CAC-NM-DND-001; 1:1000); Anti-6-4PPs (CAC-NM-DND-002; 1:1000); Validation All the antibodies listed above were validated in western blotting. ..

    Biomarker Discovery:

    Article Title: And-1 coordinates with polymerase δ to regulate nucleotide excision repair and UVB-induced skin tumorigenesis
    Article Snippet: .. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-β-Actin (CST #3700; 1:5000); Anti-GAPDH (CST #2118; 1:5000); Anti-FLAG-tag (CST #14793; 1:1000); Anti-V5-tag (CST #13202; 1:1000); Anti-Histone H3 (CST #9715; 1:1000); Anti-p125 (sc-17776; 1:1000); Anti-PCNA (sc-56; 1:1000); Anti-p66 (#21935-1-AP; 1:1000); Anti-DDB1 (A14242; 1:1000); Anti-CPDs (CAC-NM-DND-001; 1:1000); Anti-6-4PPs (CAC-NM-DND-002; 1:1000); Validation All the antibodies listed above were validated in western blotting. ..

    Western Blot:

    Article Title: And-1 coordinates with polymerase δ to regulate nucleotide excision repair and UVB-induced skin tumorigenesis
    Article Snippet: .. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-β-Actin (CST #3700; 1:5000); Anti-GAPDH (CST #2118; 1:5000); Anti-FLAG-tag (CST #14793; 1:1000); Anti-V5-tag (CST #13202; 1:1000); Anti-Histone H3 (CST #9715; 1:1000); Anti-p125 (sc-17776; 1:1000); Anti-PCNA (sc-56; 1:1000); Anti-p66 (#21935-1-AP; 1:1000); Anti-DDB1 (A14242; 1:1000); Anti-CPDs (CAC-NM-DND-001; 1:1000); Anti-6-4PPs (CAC-NM-DND-002; 1:1000); Validation All the antibodies listed above were validated in western blotting. ..

    Cell-Signaling:

    Article Title: CRY-NLRP3 complexes define a circadian checkpoint controlling inflammasome activation
    Article Snippet: .. Primary antibodies included: anti-NLRP3/NALP3 (Cryo-2, Adipogen, AG-20B-0014-C100), anti-CRY1 (ABClonal, A18028), anti-CRY2 (Bethyl, A302-615A), anti-Actin (MP Biomedicals, 0869100-CF), anti-Rev-Erbα (Cell Signaling, 13418), anti-FLAG (Sigma-Aldrich, F7425), anti-V5-Tag (Cell Signaling, 13202), anti-α-Tubulin (Sigma-Aldrich, T5168), anti-HA (Sigma-Aldrich, H6908), anti-Myc-Tag (Cell Signaling, 2276), anti-GSDMD (Abcam, ab209845; Sigma-Aldrich, G7422), anti- IL-1β (R&D Systems, AF-401-NA; Cell Signaling, 12242), anti-ASC (Adipogen, AG-25B-0006), anti-Caspase-1 (Bally-1, Adipogen, AG-20B-0048), anti-P-AMPK (Thr172, Cell Signaling, 2535T), anti-AMPK (Cell Signaling, 2532S) and anti-P-ACC1 (Cell signaling, 3662S). .. The membranes were washed, and incubated with HRP-conjugated secondary antibodies (Promega, W402B, W401B) for 1 h at room temperature.



    Similar Products

    96
    Bio-Rad mouse anti pk mab serotec mca1360
    Mouse Anti Pk Mab Serotec Mca1360, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+v5+tag/Mouse+anti+V5-Tag/pm42859192-274-123-126
    Average 96 stars, based on 1 article reviews
    mouse anti pk mab serotec mca1360 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    93
    R&D Systems anti v5 af488
    Anti V5 Af488, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+v5+tag/V5+Epitope+Tag+Alexa+Fluor%C2%AE+488-conjugated+Antibody/pm42815638-250-14-17
    Average 93 stars, based on 1 article reviews
    anti v5 af488 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc e9h80
    E9h80, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+v5+tag/V5-Tag+Mouse+mAb/pm42805951-315-26-27
    Average 95 stars, based on 1 article reviews
    e9h80 - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    Proteintech anti v5 tag
    Anti V5 Tag, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+v5+tag/V5-tag+Antibody/pm42786271-83-62-65
    Average 95 stars, based on 1 article reviews
    anti v5 tag - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    ABclonal Biotechnology ae017
    Ae017, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+v5+tag/Mouse+anti+V5-Tag+mAb/pm42690932-406-34-32
    Average 95 stars, based on 1 article reviews
    ae017 - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    96
    Bio-Rad bead 207 removal
    Bead 207 Removal, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+v5+tag/Mouse+anti+V5-Tag/10__1016_slash_j__gendis__2026__102505-85-21-26
    Average 96 stars, based on 1 article reviews
    bead 207 removal - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    95
    ABclonal Biotechnology v5 tag
    V5 Tag, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+v5+tag/Mouse+anti+V5-Tag+mAb/pm42589639-255-25-28
    Average 95 stars, based on 1 article reviews
    v5 tag - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    Fortis Life Sciences v5 epitope tag
    V5 Epitope Tag, supplied by Fortis Life Sciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+v5+tag/V5+Tag+Antibody/pm42353282-137-32-48
    Average 95 stars, based on 1 article reviews
    v5 epitope tag - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    ABclonal Biotechnology antibodies against ha tag
    Antibodies Against Ha Tag, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+v5+tag/Mouse+anti+V5-Tag+mAb/pm42304341-49-0-9
    Average 95 stars, based on 1 article reviews
    antibodies against ha tag - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    Image Search Results